Study on the Effect of Pancreatic Polypeptide on the Performance and Digestion of Meat-type Duck 添加胰多肽对肉鸭生产性能和消化机能的影响
Pancreatic acinar cells were isolated by collagenase digestion. 用胶原酶消化法分离大鼠胰腺腺泡细胞,用台盼兰染色测定细胞活力。
The functional character of dispersed pancreatic acini prepared by the method including chemical digestion, mechanical isolation and centrifugal purification was studied. 作者观察了采用化学消化、机械分离和离心提纯法制备的胰腺腺泡的机能。
Methods: Primary PSCs were obtained from pancreatic tissues in rats using the method of enzyme digestion and discontinuing density gradient centrifugation. 方法:取大鼠胰腺组织进行酶消化和不连续密度梯度离心以获得原代PSCs。
Eighteen fetuses at 12-24 weeks were selected to obtain human fetal pancreas. Human fetal pancreatic cells were gained by digestion of collagenase ⅴ. Primary culture, passage culture, the capacity of expansion and inductive differentiation in vitro were observed; 选取18例胎龄为12~24周的人胚胰腺,用Ⅴ型胶原酶消化获得贴壁生长的人胚胰腺细胞,观察其原代培养、传代培养、增殖能力及体外诱导分化能力;
In conclusion, in the larval stage, protein digestion depends on pancreatic enzymes; and when the larvae develope into the juvenile stage, pepsin plays an important role in protein digestion. 由此得出结论,(1)在仔鱼期,蛋白质的消化是依靠胰腺酶进行的,进入稚鱼期,胃蛋白酶开始对蛋白质的消化起重要作用;
Methods: Collagenase solution was injected through the pancreatic duct. The splenic lobe of porcine pancreas was digested by static digestion combined with physics digestion. 方法:采用胶原酶胰导管注射负荷技术,静止消化与物理消化相结合消化分离成年猪胰腺脾叶;
A method for the in vitro production of newborn porcine pancreatic islellike cell clusters ( ICC) by collagenase digestion and tissue culture was introduced in this paper. 本文介绍一种以国产胶原酶消化与组织培养获得新生猪胰岛样细胞团(ICC)的方法。
Rat pancreatic islets were separated by intraductal collagenase injection and stationary digestion and were purified by dextran discontinuous density gradient. 为探讨胰岛移植物的制备方法,采用胰管注射胶原酶、胰腺静止消化方法,分离成年大鼠胰岛,葡聚糖离心纯化。
Method 1. Isolation and purification of islet cells: With our own method, pancreatic tissue was enzymatically digested by two-step digestion method and purified by discontinuous gradient centrifugation on four layers of HC-A-Ficoll solutions by tube method. 方法:1、胰岛细胞的分离纯化:按本实验室建立的方法,用胶原酶P两步法消化分离胰腺,用试管法通过不连续密度梯度HC-A-Ficoll纯化液纯化胰岛细胞。
Rat pancreatic islets were microencapsulated with, sodium alginate and poly-lysine, according to Sun's method after being isolated from pancreatic tissue using modified collagenase digestion and Ficoll density gradient separation. 用改良胶原酶消化技术和聚蔗糖密度梯度离心方法将大鼠胰岛从胰腺分离,按Sun等的方法用海藻酸和聚赖氨酸等予以微囊化。
Pancreatic triglyceride lipase ( PTL), is a member of lipase family, plays very important roles in the digestion and absorption of lipids. 胰甘油三酯脂酶(pancreatictriglyceridelipase,PTL),是甘油三酯脂酶家族的一个成员,在脂质的消化与吸收中发挥非常重要的作用。